Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures
Interactions with RNA-binding proteins (RBPs) are integral to RNA perform and mobile regulation, and dynamically replicate particular mobile situations. Nevertheless, presently obtainable instruments for predicting RBP-RNA interactions make use of RNA sequence and/or predicted RNA constructions, and due to this fact don’t seize their condition-dependent nature. Right here, after profiling transcriptome-wide in vivo RNA secondary constructions in seven cell varieties, we developed PrismNet, a deep studying device that integrates experimental in vivo RNA construction knowledge and RBP binding knowledge for matched cells to precisely predict dynamic RBP binding in varied mobile situations. PrismNet outcomes for 168 RBPs assist its utility for each understanding CLIP-seq outcomes and largely extending such interplay knowledge to precisely analyze extra cell varieties.
Additional, PrismNet employs an “consideration” technique to computationally establish actual RBP-binding nucleotides, and we found enrichment amongst dynamic RBP-binding websites for structure-changing variants (riboSNitches), which may hyperlink genetic ailments with dysregulated RBP bindings. Our wealthy profiling knowledge and deep learning-based prediction device present entry to a beforehand inaccessible layer of cell-type-specific RBP-RNA interactions, with clear utility for understanding and treating human ailments.
Actual-Time Imaging of Polioviral RNA Translocation throughout a Membrane
Genome switch from a virus right into a cell is a vital early step in viral replication. Enveloped viruses obtain the supply of their genomes into the cytoplasm by merging the viral membrane with the mobile membrane through a conceptually easy mechanism referred to as membrane fusion. In distinction, genome translocation mechanisms in nonenveloped viruses, which lack viral membranes, stay poorly understood.
Though mobile assays present helpful details about cell entry and genome launch, it’s tough to acquire detailed mechanistic insights due each to the inherent technical difficulties related to direct visualization of those processes and to the prevalence of nonproductive occasions in mobile assays carried out at a really excessive multiplicity of an infection. To beat these points, we developed an in vitro single-particle fluorescence assay to characterize genome launch from a nonenveloped virus (poliovirus) in actual time utilizing a tethered receptor-decorated liposome system.
Our outcomes recommend that poliovirus genome launch is a fancy course of that consists of a number of rate-limiting steps. Apparently, we discovered that the addition of exogenous wild-type capsid protein VP4, however not mutant VP4, enhanced the effectivity of genome translocation. These outcomes, along with prior structural evaluation, recommend that VP4 interacts with RNA instantly and types a protecting, membrane-spanning channel throughout genome translocation.
Moreover, our knowledge point out that VP4 dynamically interacts with RNA, moderately than forming a static tube for RNA translocation. This examine gives new insights into poliovirus genome translocation and presents a cell-free assay that may be utilized broadly to research genome launch processes in different nonenveloped viruses.IMPORTANCE The preliminary switch of genomic materials from a virus into a bunch cell is a key step in any viral an infection. Consequently, understanding how viruses ship their genomes into cells may reveal engaging therapeutic targets.

Though typical biochemical and mobile assays have offered helpful details about cell entry, the mechanism used to ship the viral genomes throughout the mobile membrane into the cytoplasm is just not properly characterised for nonenveloped viruses corresponding to poliovirus. On this examine, we developed a fluorescence imaging assay to visualise poliovirus genome launch utilizing an artificial vesicle system. Our outcomes not solely present new mechanistic insights into poliovirus genome translocation but in addition provide a cell-free assay to bridge gaps in understanding of this course of in different nonenveloped viruses.
Towards a Complete Evaluation of Posttranscriptional Regulatory Networks: a New Device for the Identification of Small RNA Regulators of Particular mRNAs
Numerous computational or experimental instruments have been developed to establish targets of small RNA (sRNA) regulation. Right here, we modified considered one of these strategies, primarily based on in vivo proximity ligation of sRNAs sure to their targets, known as rGRIL-seq, that can be utilized to seize sRNA regulators of a gene of curiosity. Intracellular expression of bacteriophage T4 RNA ligase results in a covalent linking of sRNAs base-paired with mRNAs, and the chimeras are captured utilizing oligonucleotides complementary to the mRNA, adopted by sequencing.
This permits the identification of identified in addition to novel sRNAs. We utilized rGRIL-seq towards discovering sRNA regulators of expression of the stress response sigma issue RpoS in Escherichia coli, Pseudomonas aeruginosa, and Vibrio cholerae In E. coli, we confirmed the regulatory function of identified sRNAs and found a brand new unfavorable regulator, asYbiE. When utilized to P. aeruginosa and V. cholerae, we recognized two novel sRNAs (s03661 and s0223) in P. aeruginosa and two identified sRNAs (TfoR and Vcr043) in V. cholerae as direct regulators of rpoS The usage of rGRIL-seq for outlining a number of posttranscriptional regulatory inputs into particular person mRNAs represents a step towards a extra complete understanding of the workings of bacterial regulatory networks.
IMPORTANCE With the popularity of the significance of posttranscriptional regulation mediated by bacterial small RNAs (sRNAs), their contribution to international gene expression regulatory networks must be addressed in a very complete method. Whereas a single sRNA targets a number of RNAs, an mRNA might be regulated by a number of sRNAs that may be both transcribed individually or derived by processing of mRNAs. On this paper, we developed a device (known as rGRIL-seq) to establish sRNAs that regulate mRNAs no matter their origin.
Total RNA - Lupus: Pancreas |
R1236188Lup-50 |
Biochain |
50 ug |
EUR 421.2 |
RNA isolater Total RNA Extraction Reagent |
R401-01 |
Vazyme |
100 ml |
EUR 159.6 |
Total RNA - hypertension: Heart |
R1236122Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
rBAC MINI TOTAL RNA KIT |
IB47420 |
IBI Scientific |
4 PREP SAMPLE KIT |
EUR 30.11 |
Description: (FR) [*] |
rBAC MINI TOTAL RNA KIT |
IB47421 |
IBI Scientific |
100 PREP KIT |
EUR 611.55 |
Description: (FR) [*] |
rBAC MINI TOTAL RNA KIT |
IB47422 |
IBI Scientific |
300 PREP KIT |
EUR 1339.49 |
Description: (FR) [*] |
rYEAST MINI TOTAL RNA KIT |
IB47410 |
IBI Scientific |
4 PREP SAMPLE KIT |
EUR 30.11 |
Description: [*] |
rYEAST MINI TOTAL RNA KIT |
IB47411 |
IBI Scientific |
100 PREP KIT |
EUR 615.63 |
Description: [*] |
rYEAST MINI TOTAL RNA KIT |
IB47412 |
IBI Scientific |
300 PREP KIT |
EUR 1627.73 |
Description: [*] |
Total RNA Isolation Kit II |
FATRS--001 |
Favorgen |
100 preps |
EUR 369.6 |
Total RNA Isolation Kit II |
FATRS--050 |
Favorgen |
50 preps |
EUR 259.2 |
MINI TOTAL RNA KIT-PLANT |
IB47340 |
IBI Scientific |
4 PREP SAMPLE KIT |
EUR 30.42 |
MINI TOTAL RNA KIT-PLANT |
IB47341 |
IBI Scientific |
50 PREP KIT |
EUR 336.32 |
MINI TOTAL RNA KIT-PLANT |
IB47342 |
IBI Scientific |
100 PREP KIT |
EUR 579.66 |
MINI TOTAL RNA KIT-PLANT |
IB47343 |
IBI Scientific |
300 PREP KIT |
EUR 1415.08 |
MINI TOTAL RNA KIT-TISSUE |
IB47300 |
IBI Scientific |
4 PREP SAMPLE KIT [*] |
EUR 29.82 |
MINI TOTAL RNA KIT-TISSUE |
IB47301 |
IBI Scientific |
50 PREP KIT |
EUR 307.4 |
MINI TOTAL RNA KIT-TISSUE |
IB47302 |
IBI Scientific |
100 PREP KIT |
EUR 574.01 |
MINI TOTAL RNA KIT-TISSUE |
IB47303 |
IBI Scientific |
300 PREP KIT |
EUR 1368.18 |
Total RNA Isolation Kit (Plant) |
P-9104 |
EpiGentek |
50 samples |
EUR 416.7 |
Total RNA Isolation Kit (Tissue) |
P-9102 |
EpiGentek |
50 samples |
EUR 416.7 |
Total RNA - Liver Cirrhosis: Lung |
R1236152Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Colon |
R1236090Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Liver |
R1236149Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Lupus: Small Intestine |
R1236226Lup-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Alzheimer's Disease: Pons |
R1236071Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - Diabetic Disease: Colon |
R1236090Dia-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Kidney |
R1236142Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Rectum |
R1236206Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Spleen |
R1236246Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
AnaPrep Total RNA Extraction Kit |
Z1322015 |
Biochain |
1 kit (48 extractions) Including all required plastic disposables |
EUR 246 |
Total RNA - Alzheimer's Disease: Brain |
R1236035Alz-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Pancreas |
R1236188Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Diabetic Disease: Stomach |
R1236248Dia-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Diaphragm |
R1236169Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Diabetic Disease: Pancreas |
R1236188Dia-50 |
Biochain |
50 ug |
EUR 421.2 |
xTractPure Total RNA extraction Kit |
Z2212007 |
Biochain |
1 kit |
EUR 499 |
TOTAL RNA KIT (PLANT)-96 WELL |
IB47380 |
IBI Scientific |
2X96 PREP KIT |
EUR 954.22 |
TOTAL RNA KIT (PLANT)-96 WELL |
IB47381 |
IBI Scientific |
4X96 PREP KIT |
EUR 1728.67 |
TOTAL RNA KIT (PLANT)-96 WELL |
IB47382 |
IBI Scientific |
10X96 PREP KIT |
EUR 3886.07 |
Total RNA - Diabetic Disease: Esophagus |
R1236106Dia-50 |
Biochain |
50 ug |
EUR 421.2 |
MINI TOTAL RNA KIT-BLOOD/CC |
IB47320 |
IBI Scientific |
4 PREP SAMPLE KIT |
EUR 30.42 |
MINI TOTAL RNA KIT-BLOOD/CC |
IB47321 |
IBI Scientific |
50 PREP KIT |
EUR 336.32 |
MINI TOTAL RNA KIT-BLOOD/CC |
IB47322 |
IBI Scientific |
100 PREP KIT |
EUR 569.41 |
MINI TOTAL RNA KIT-BLOOD/CC |
IB47323 |
IBI Scientific |
300 PREP KIT |
EUR 1526.96 |
Plant Total RNA Mini Kit (50prep) |
FAPRK-001 |
Favorgen |
50 preps |
EUR 198 |
Plant Total RNA Mini Kit (100prep) |
FAPRK-001-1 |
Favorgen |
100 preps |
EUR 259.2 |
Plant Total RNA Mini Kit (300prep) |
FAPRK-001-2 |
Favorgen |
300 preps |
EUR 484.8 |
Plant Total RNA Maxi Kit (10prep) |
FAPRK-002 |
Favorgen |
10 preps |
EUR 241.2 |
Plant Total RNA Maxi Kit (24prep) |
FAPRK-002-1 |
Favorgen |
24 preps |
EUR 380.4 |
Total RNA ExtractionMini KitPlant Tissues |
FYG303-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Total RNA ExtractionMini KitPlant Tissues |
FYG303-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
Total RNA ExtractionMini KitPlant Tissues |
FYG303-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
Tissue Total RNA Mini Kit (50prep) |
FATRK-001 |
Favorgen |
50 preps |
EUR 198 |
Tissue Total RNA Mini Kit (100prep) |
FATRK-001-1 |
Favorgen |
100 preps |
EUR 259.2 |
Tissue Total RNA Mini Kit (300prep) |
FATRK-001-2 |
Favorgen |
300 preps |
EUR 484.8 |
Tissue Total RNA Maxi Kit (10prep) |
FATRK-003 |
Favorgen |
10 preps |
EUR 241.2 |
Tissue Total RNA Maxi Kit (24prep) |
FATRK-003-1 |
Favorgen |
24 preps |
EUR 380.4 |
Total RNA - Rat Normal Tissue: Lung |
R1434152-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA ExtractionMini KitAnimal Tissues |
FYG301-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
Total RNA ExtractionMini KitAnimal Tissues |
FYG302-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Total RNA ExtractionMini KitAnimal Tissues |
FYG302-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
Total RNA - Human Tumor Tissue: Bone |
R1235023-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Lung |
R1235152-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Nose |
R1235178-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Rat Normal Tissue: Brain |
R1434035-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Colon |
R1434090-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Heart |
R1434122-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Liver |
R1434149-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Ovary |
R1434183-50 |
Biochain |
50 ug |
EUR 313 |
Total RNA - Human Tumor Tissue: Colon |
R1235090-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Liver |
R1235149-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Ovary |
R1235183-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Mouse Normal Tissue: Lung |
R1334152-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Kidney |
R1434142-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Rectum |
R1434206-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Rat Normal Tissue: Spleen |
R1434246-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Testis |
R1434260-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Thymus |
R1434264-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Plant Normal Tissue: Corn |
R1634330 |
Biochain |
100 ug |
EUR 646 |
Total RNA - Plant Normal Tissue: Rice |
R1634360 |
Biochain |
100 ug |
EUR 646 |
Broad Range Total RNA Isolation Kit |
K1341050 |
Biochain |
1 kit |
EUR 259 |
Total RNA - Human Tumor Tissue: Breast |
R1235086-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Kidney |
R1235142-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Rectum |
R1235206-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Thymus |
R1235264-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Tonsil |
R1235268-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Uterus |
R1235274-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Mouse Normal Tissue: Brain |
R1334035-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Mouse Normal Tissue: Colon |
R1334090-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Mouse Normal Tissue: Heart |
R1334122-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Mouse Normal Tissue: Liver |
R1334149-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Adipose |
R1434003-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Rat Normal Tissue: Bladder |
R1434010-50 |
Biochain |
50 ug |
EUR 322 |
Total RNA - Rat Normal Tissue: Stomach |
R1434248-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Plant Normal Tissue: Wheat |
R1634390 |
Biochain |
100 ug |
EUR 646 |
Zenoquick Whole Blood Total RNA Kit |
Z5010-050 |
GenDepot |
50 preps |
EUR 578.4 |
Zenoquick Whole Blood Total RNA Kit |
Z5010-100 |
GenDepot |
2x50 preps |
EUR 967.2 |
EpiQuik Total RNA Isolation Fast Kit |
P-9105-050 |
EpiGentek |
50 samples |
EUR 176 |
Total RNA - Human Tumor Tissue: Adrenal |
R1235004-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Bladder |
R1235010-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Parotid |
R1235190-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Stomach |
R1235248-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Human Tumor Tissue: Thyroid |
R1235265-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Mouse Normal Tissue: Kidney |
R1334142-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Mouse Normal Tissue: Rectum |
R1334206-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Mouse Normal Tissue: Spleen |
R1334246-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Mouse Normal Tissue: Testis |
R1334260-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Rat Normal Tissue: Pancreas |
R1434188-50 |
Biochain |
50 ug |
EUR 313 |
Total RNA - Rat Normal Tissue: Placenta |
R1434200-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Plant Normal Tissue: Orange |
R1634340 |
Biochain |
100 ug |
EUR 646 |
Total RNA - Plant Normal Tissue: Potato |
R1634350 |
Biochain |
100 ug |
EUR 646 |
Total RNA - hypertension: Interventricular Septum |
R1236130Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
Rapid Animal Total RNA Extraction Kit |
AT4181 |
Bio Basic |
50Preps |
EUR 105.67 |
|
Rapid Animal Total RNA Extraction Kit |
AT4182 |
Bio Basic |
250preps |
EUR 242.7 |
|
Total RNA - Human Tumor Tissue: Melanoma |
R1235218A-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Mouse Normal Tissue: Adipose |
R1334003-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA - Mouse Normal Tissue: Bladder |
R1334010-50 |
Biochain |
50 ug |
EUR 322 |
Total RNA - Mouse Normal Tissue: Stomach |
R1334248-50 |
Biochain |
50 ug |
EUR 130 |
Total RNA - Human Tumor Tissue: Esophagus |
R1235106-50 |
Biochain |
50 ug |
EUR 400 |
Total RNA - Mouse Normal Tissue: Pancreas |
R1334188-50 |
Biochain |
50 ug |
EUR 369 |
Total RNA - Mouse Normal Tissue: Placenta |
R1334200-50 |
Biochain |
50 ug |
EUR 209 |
Total RNA Plus Mini Kit (50 prep) |
FATRK-P-050 |
Favorgen |
50 preps |
EUR 214.8 |
Total RNA Plus Mini Kit (100 prep) |
FATRK-P-100 |
Favorgen |
100 preps |
EUR 290.4 |
Total RNA - Alzheimer's Disease: Frontal Lobe |
R1236051Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - Congenital heart disease: Heart |
R1236122Hd-3 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - hypertension: Heart Atrium, left |
R1236126Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
96-well Total RNA Kit (1 plates) |
FATRE-96001 |
Favorgen |
1 prep |
EUR 232.8 |
96-well Total RNA Kit (2 plates) |
FATRE-96002 |
Favorgen |
2 preps |
EUR 337.2 |
96-well Total RNA Kit (4 plates) |
FATRE-96004 |
Favorgen |
4 preps |
EUR 537.6 |
Total RNA - Alzheimer's Disease: Parietal Lobe |
R1236066Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - Alzheimer's Disease: Temporal Lobe |
R1236078Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - hypertension: Heart Atrium, right |
R1236127Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Liver Cirrhosis: Small Intestine |
R1236226Lcs-50 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Plant Normal Tissue: Arabidopsis |
R1634310 |
Biochain |
100 ug |
EUR 646 |
Total RNA - Alzheimer's Disease: Occipital Lobe |
R1236062Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - Human Tumor Tissue: Synoviosarcoma |
R1235257-10 |
Biochain |
10 ug |
EUR 400 |
Total RNA - Alzheimer's Disease: Corpus Callosum |
R1236045Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - hypertension: Heart Ventricle, left |
R1236138Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Human Tumor Cell Line: A431 |
R1255801-50 |
Biochain |
50 ug |
EUR 191 |
Total RNA - Human Tumor Cell Line: Hela |
R1255811-50 |
Biochain |
50 ug |
EUR 191 |
Total RNA - Human Tumor Cell Line: Raji |
R1255840-50 |
Biochain |
50 ug |
EUR 191 |
Total RNA - Alzheimer's Disease: Precentral Gyrus |
R1236073Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - hypertension: Heart Ventricle, right |
R1236139Hd-2 |
Biochain |
50 ug |
EUR 421.2 |
Total RNA - Alzheimer's Disease: Postcentral Gyrus |
R1236072Alz-10 |
Biochain |
10 ug |
EUR 421.2 |
Total RNA - Human Tumor Cell Line: Jurkat |
R1255815-50 |
Biochain |
50 ug |
EUR 191 |
Total RNA - Plant Normal Tissue: Soy bean |
R1634370 |
Biochain |
100 ug |
EUR 646 |
Total RNA ExtractionMini KitBlood/Cultured Cell |
FYG301-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
Total RNA - Mouse Normal Tissue: Whole Eye |
R1334108-50 |
Biochain |
50 ug |
EUR 313 |
Total RNA - Human Adult Normal Tissue: Lung |
R1234152-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Skin |
R1234218-50 |
Biochain |
50 ug |
EUR 284 |
Total RNA - Human Adult Normal Tissue: Brain |
R1234035-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Cecum |
R1234089-10 |
Biochain |
10 ug |
EUR 183 |
Total RNA - Human Adult Normal Tissue: Colon |
R1234090-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Heart |
R1234122-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Liver |
R1234149-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Ovary |
R1234183-10 |
Biochain |
10 ug |
EUR 643 |
Total RNA - Human Adult Normal Tissue: Penis |
R1234194-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Breast |
R1234086-50 |
Biochain |
50 ug |
EUR 284 |
Total RNA - Human Adult Normal Tissue: Kidney |
R1234142-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Rectum |
R1234206-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Spleen |
R1234246-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Testis |
R1234260-50 |
Biochain |
50 ug |
EUR 170 |
Total RNA - Human Adult Normal Tissue: Throat |
R1234263-10 |
Biochain |
10 ug |
EUR 191 |
Total RNA - Human Adult Normal Tissue: Thymus |
R1234264-10 |
Biochain |
10 µg |
EUR 643 |
Total RNA - Human Adult Normal Tissue: Tongue |
R1234267-50 |
Biochain |
50 ug |
EUR 170 |
We demonstrated the utility of this strategy by figuring out constructive and unfavorable sRNA regulators of the rpoS mRNA in three bacterial species. We not solely described identified sRNAs of E. coli or P. aeruginosa that management rpoS but in addition recognized a number of new rpoS regulators in V. cholerae Due to this fact, rGRIL-seq can be utilized to establish species-specific sRNAs concentrating on a conserved mRNA, they usually seemingly play an necessary function in bacterial adaptation to particular environmental niches.
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